Composite

Part:BBa_K2966007:Design

Designed by: Julianne Dejoie   Group: iGEM19_Purdue   (2019-10-16)


Pc-NodC_Pf


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to low AT concentration, we had to take the T4 terminator which changed the RE cut sites for this vector alone.


Source

Wilton, R., Ahrendt, A. J., Shinde, S., Sholto-Douglas, D. J., Johnson, J. L., Brennan, M. B., & Kemner, K. M. (2018, February 1). A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of Root Colonizing Pseudomonads. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/29449848.

http://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase

References